Human GJA1 / CX43 / Connexin 43

Rabbit

Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)

Bat, Horse, Chicken, Xenopus (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic 16 amino acid peptide from C-Terminus of human GJA1 / Connexin 43. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Rabbit, Pig, Opossum, Guinea pig (100%); Bat, Horse, Chicken, Lizard, Xenopus (94%); Trout, Salmon (88%); Zebrafish, Sea anemone, Nematode (81%).

C-Terminus

Human GJA1 / Connexin 43. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

• IHC
• IHC - Paraffin (10 - 20 µg/ml)

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10-20 ug/ml.

PBS, 0.1% Sodium Azide

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P17302 NCBI: NM_000165 NP_000156.1