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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154312-100 100 µg (1 mg/ml) $355 
GFP Antibody - Immunofluorescence Microscopy of chicken anti-GFP antibody. Tissue: KruppleGAL4 driver line in Drosophila eye disc. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-GFP antibody diluted 1:500 for 2 hr at RT. Secondary antibody: Alexa™488 conjugated anti-Chicken IgG at 1:300 for 1 hr at RT.5% NGS in PBS with 0.1% Triton X-100 for 15 min. Localization: E2F-1 pS364 is nuclear and occasionally cytoplasmic. Staining: recombinant tau-myc-GFP protein as green fluorescent signal.
GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 ug of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kD band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 ug/ml, followed by washes and reaction with a 1:20000 dilution of IRDye800 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] MX10 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
GFP Antibody - Immunofluorescence Microscopy of chicken anti-GFP antibody. Tissue: KruppleGAL4 driver line in Drosophila eye disc. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-GFP antibody diluted 1:500 for 2 hr at RT. Secondary antibody: Alexa™488 conjugated anti-Chicken IgG at 1:300 for 1 hr at RT.5% NGS in PBS with 0.1% Triton X-100 for 15 min. Localization: E2F-1 pS364 is nuclear and occasionally cytoplasmic. Staining: recombinant tau-myc-GFP protein as green fluorescent signal.
GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP protein detected with polyclonal anti-GFP antibody. Lane 1 shows negative control staining of 20 ug of mouse spleen lysate. Lane 2 shows staining of mouse spleen lysate spiked with 50 ng of wt GFP. This antibody detects a 27 kD band corresponding to the GFP epitope tag commonly used in recombinant constructs. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE followed by transfer to nitrocellulose using standard methods. After blocking with 5% BSA in PBS, the membrane was probed for 2 h at room temperature with the primary antibody diluted in 5% BSA to 2 ug/ml, followed by washes and reaction with a 1:20000 dilution of IRDye800 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] MX10 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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GFP Antibody LS‑C154312

GFP Antibody LS‑C154312

Antibody:
Chicken Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C154312-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Chicken Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified

Specifications

Description
GFP antibody LS-C154312 is an unconjugated chicken polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 1 publication.
Target
Aequorea victoria GFP
Host
Chicken
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Immunofluorescence
  • Western blot (1:200 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Usage
Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluor-escence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

Publications (1)

The role of PSD-95 and cypin in morphological changes in dendrites following sublethal NMDA exposure. Tseng CY, Firestein BL. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:15468-80.

Customer Reviews (0)


Requested From: United States
Date Requested: 11/13/2019