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GFP Antibody (clone 9F9.F9, Biotin) LS‑C154212

Catalog Number Size Price
LS-C154212-1 1 mg (1 mg/ml) $710 
Biotin Mouse Anti-GFP Antibody - Western Blot. Western Blot showing detection of Green Fluorescent Protein. 50 ng of GFP (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-GFP (MOUSE) Monoclonal Antibody Biotin Conjugated Anti-GFP (MOUSE) Monoclonal Antibody Biotin Conjugated - 600-306-215 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 28 kD molecular weight position expected for GFP. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GFP Antibody (clone 9F9.F9, Biotin) LS‑C154212

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified
Price
Catalog Number
$710 
LS-C154212-1
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Biotin, Unmodified

Specifications

Description
GFP antibody LS-C154212 is a biotin-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Target
Aequorea victoria GFP
Host
Mouse
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
9F9.F9
Conjugations
Biotin
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The immunogen is a GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Usage
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute with 1ml of distilled H2O.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Requested From: United States
Date Requested: 8/18/2019
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