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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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GFP Antibody (clone 9F9.F9, AP) LS‑C154211

Catalog Number Size Price
LS-C154211-1 1 mg (1 mg/ml) $845 
GFP Antibody Alkaline Phosphatase Conjugated Western Blot. Western Blot of Mouse anti-GFP Antibody Alkaline Phosphatase Conjugated Lane 1: GFP Load: 100 ng per lane Secondary antibody: GFP Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 28 kD, 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GFP Antibody (clone 9F9.F9, AP) LS‑C154211

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Alkaline Phosphatase, Unmodified
Price
Catalog Number
$845 
LS-C154211-1
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
GFP antibody LS-C154211 is an AP-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Target
Aequorea victoria GFP
Host
Mouse
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
9F9.F9
Conjugations
Alkaline Phosphatase
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The immunogen is a GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Usage
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.01% sodium azide, sterile filtered
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Requested From: United States
Date Requested: 8/24/2019
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