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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154196-1 1 mg (1 mg/ml) $620 
GFP Antibody - Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody. Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GFP Antibody (AP) LS‑C154196

GFP Antibody (AP) LS‑C154196

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Alkaline Phosphatase, Unmodified
Price
Catalog Number
$620 
LS-C154196-1
1 mg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, WB, ELISA
Reactivity:
Aequorea victoria
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
GFP antibody LS-C154196 is an AP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Target
Aequorea victoria GFP
Host
Goat
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Usage
Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50% glycerol, 1% BSA, 0.1% sodium azide, sterile filtered
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Requested From: United States
Date Requested: 11/13/2019