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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GDNF Antibody (aa8‑36) LS‑C166432 Best Seller
GDNF antibody LS-C166432 is an unconjugated rabbit polyclonal antibody to GDNF from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C166432-400
400 µl
$305

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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

GDNF antibody LS-C166432 is an unconjugated rabbit polyclonal antibody to GDNF from human and mouse. Validated for Flow, IHC and WB.

Specifications

Target
Human GDNF
Synonyms
GDNF, HSCR3, HFB1-GDNF, ATF, HGDNF
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9718 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166432. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa8-36
Specificity
This GDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human GDNF.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About GDNF
GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. P39905 NM_000514 NP_000505.1


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Images

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.
Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.

Flow Cytometry

GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.
Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.

Flow Cytometry

GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.
Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.

Flow Cytometry

GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GDNF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.
Western blot of GDNF Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). GDNF (arrow) was detected using the purified antibody.

Flow Cytometry

GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GDNF Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/14/2018

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