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Catalog Number Size Price
LS-C745241-25 25 µl (1.37 mg/ml) $304 
GDF15 Antibody - Western blot using the affinity purified anti-Human NAG-1 antibody shows detection of a 42 kDa band corresponding to purified recombinant fusion protein MBP-human NAG-1.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1:1000 in 1% BSA-TBST overnight at 4°C. DyLight649 Goat Anti-Rabbit IgG secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Human GDF15 Antibody (WB) LS‑C745241

Polyclonal Rabbit anti‑Human GDF15 Antibody (WB) LS‑C745241

Antibody:
GDF15 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C745241-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GDF15 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
GDF15 antibody LS-C745241 is an unconjugated rabbit polyclonal antibody to GDF15 from human. It is reactive with human and mouse. Validated for ELISA and WB.
Target
Human GDF15
Synonyms
GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein.  Cross reactivity to MBP was removed via cross-adsorption chromatography.
Specificity
This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100% and 98% homology, respectively, and with macaque based on an 94% homology, and with mouse and rat based on 70% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Applications
  • Western blot (1:800 - 1:1500)
  • ELISA (1:500)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GDF15
Q99988 NM_004864 NP_004855.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024