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Catalog Number Size Price
LS-C744826-25 25 µl (1.13 mg/ml) $275 
GDF15 Antibody - Western blot using the anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag, lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag, lane 3 - yeast cell lysate expressing NAG-1 H variant and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins correspond to 32 kDa and 16 kDa bands as indicated by the arrowheads. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Detection occurred using a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody in Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System. Other detection systems will yield similar results.

Monoclonal Mouse anti‑Human GDF15 Antibody (clone 23B3.D2.H5, WB) LS‑C744826

Monoclonal Mouse anti‑Human GDF15 Antibody (clone 23B3.D2.H5, WB) LS‑C744826

Antibody:
GDF15 Mouse anti-Human Monoclonal (23B3.D2.H5) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744826-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GDF15 Mouse anti-Human Monoclonal (23B3.D2.H5) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GDF15 antibody LS-C744826 is an unconjugated mouse monoclonal antibody to human GDF15. Validated for ELISA and WB.
Target
Human GDF15
Synonyms
GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
23B3.D2.H5
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
This Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.
Specificity
This antibody reacts with the C-terminus of endogenous NAG-1 protein from human tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • ELISA (1:2000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GDF15
Q99988 NM_004864 NP_004855.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021