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Catalog Number Size Price
LS-C750034-20 20 µl $253 
LS-C750034-50 50 µl $279 
LS-C750034-100 100 µl $333 
LS-C750034-200 200 µl $429 
GCK / Glucokinase Antibody - Western blot analysis of extracts of various cell lines, using GCK antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.

Polyclonal Rabbit anti‑Human GCK / Glucokinase Antibody (IHC, WB) LS‑C750034

Polyclonal Rabbit anti‑Human GCK / Glucokinase Antibody (IHC, WB) LS‑C750034

Antibody:
GCK / Glucokinase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C750034-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
GCK / Glucokinase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Glucokinase antibody LS-C750034 is an unconjugated rabbit polyclonal antibody to Glucokinase (GCK) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human GCK / Glucokinase
Synonyms
GCK | FGQTL3 | Glucokinase | HK IV | HKIV | Hexokinase d, liver isozymes | Hexokinase-4 | Hexokinase-D | HXKP | GLK | Glucokinase (hexokinase 4) | Hexokinase type IV | HHF3 | HK4 | LGLK | MODY2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human GCK (NP_000153.1). RMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 52kDa, while the observed MW by Western blot was 80/55kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GCK / Glucokinase
P35557 NM_000162 NP_000153.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021