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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GALC / Galactocerebrosidase Antibody (aa324‑373) LS‑C102145
Galactocerebrosidase antibody LS-C102145 is an unconjugated rabbit polyclonal antibody to Galactocerebrosidase (GALC) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102145-50
50 µg
$365

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Product Description

Galactocerebrosidase antibody LS-C102145 is an unconjugated rabbit polyclonal antibody to Galactocerebrosidase (GALC) from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human GALC / Galactocerebrosidase
Synonyms
GALC, Galactocerebrosidase, GALCERase, Galactosylceramidase, Galactosylceraminidase
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
Synthetic peptide located between aa324-373 of human GALC (P54803, NP_000144). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse (100%); Bovine, Platypus, Xenopus (92%); Opossum, Guinea pig (85%); Stickleback, Medaka, Pufferfish (84%).
Epitope
aa324-373
Specificity
Human GALC
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GALC / Galactocerebrosidase
Galactocerebrosidase (GALC) is a lysosomal enzyme that hydrolyzes several galactolipids. GALC deficiency is associated with Krabbe disease (globoid cell leukodystrophy). The gene is about 60 kb in length and consists of 17 exons. This gene contains ten GC-box-like sequences within the promoter region but no typical TATA box. This feature is also characteristic of other lysosomal protein encoding genes. P54803 NM_000153 NP_000144.2


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Images

Western blot

GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GALC antibody Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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