GABRA3 antibody LS-B4146 is an unconjugated goat polyclonal antibody to GABRA3 from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Dog, Chicken (at least 90% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
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GABRA3 antibody was raised against synthetic peptide NRESAIKGMIRKQ from C-Terminus of human GABRA3 (NP_000799.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Platypus (100%); Panda, Dog, Chicken (92%); Turkey (85%).
Immunohistochemistry: LS-B4146 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4146 was determined to be 2.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.