Human FZD3 / Frizzled 3

Rabbit

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic 18 amino acid peptide from N-Terminus extracellular domain of human FZD3 / Frizzled 3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%); Xenopus (89%); Platypus, Zebrafish (83%).

N-Terminus

Human FZD3 / Frizzled 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD6 (39%).

• IHC
• IHC - Paraffin (3 - 5 µg/ml)

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 3-5 ug/ml.

PBS, 0.1% Sodium Azide

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NPG1 NCBI: NM_017412 NP_059108.1