Mouse FZD3 / Frizzled 3

Rat

Mouse, Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rmFrizzled-3 extracellular CRD domain.

Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 or -8. Species cross-reactivity: Human.

• IHC
• IHC - Frozen
• Western blot
• Flow Cytometry
• ELISA

Suitable for use in Direct ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. By Flow Cytometry and Immunohistochemistry, this antibody detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Flow Cytometry: Dilute this antibody to 5-10 ug/ml and add 10 ml of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: A biotin conjugate of this antibody at a concentration of 8 ug/ml was used to detect Frizzled-3 in frozen mouse embryo sections. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-3. Using a colorimetric detection system, the detection limit for rmFrizzled-3 is ~1 ng/lane under non-reducing and reducing conditions.

Lyophilized from PBS, 5% Trehalose

Reconstitute in 200 ul sterile PBS

Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NPG1 NCBI: NM_017412 NP_059108.1