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Catalog Number Size Price
LS-C23709-100 100 µg $747 
Antibody

Monoclonal Rat anti‑Mouse FZD3 / Frizzled 3 Antibody (Biotin, IHC, WB) LS‑C23709

Monoclonal Rat anti‑Mouse FZD3 / Frizzled 3 Antibody (Biotin, IHC, WB) LS‑C23709

Antibody:
FZD3 / Frizzled 3 Rat anti-Mouse Monoclonal (Biotin) Antibody
Application:
IHC, IHC-Fr, ICC, WB, Flo
Reactivity:
Mouse, Human
Format:
Biotin, Unmodified
Price
Catalog Number
$747
LS-C23709-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FZD3 / Frizzled 3 Rat anti-Mouse Monoclonal (Biotin) Antibody
Application:
IHC, IHC-Fr, ICC, WB, Flo
Reactivity:
Mouse, Human
Format:
Biotin, Unmodified

Specifications

Description
Frizzled 3 antibody LS-C23709 is a biotin-conjugated rat monoclonal antibody to Frizzled 3 (FZD3) from mouse. It is reactive with human and mouse. Validated for Flow, ICC, IHC and WB.
Target
Mouse FZD3 / Frizzled 3
Synonyms
FZD3 | Frizzled family receptor 3 | Frizzled 3 | HFz3 | Frizzled homolog 3 | Fz-3 | Wnt receptor frizzled-3 | Frizzled-3
Host
Rat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Biotin
Purification
Protein G purified
Modifications
Unmodified
Immunogen
NS0-derived rmFrizzled-3 extracellular CRD.
Specificity
Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 ug/ml to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, BSA
Reconstitution
Reconstitute with 1 ml sterile PBS.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FZD3 / Frizzled 3
Q9NPG1 NM_017412 NP_059108.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/13/2022