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| Catalog Number | Size | Price |
|---|---|---|
| LS-C6894-100 | 100 µg | $930 |
Monoclonal Rat anti‑Mouse FZD1 / Frizzled 1 Antibody (WB) LS‑C6894
Monoclonal Rat anti‑Mouse FZD1 / Frizzled 1 Antibody (WB) LS‑C6894
Antibody:
FZD1 / Frizzled 1 Rat anti-Mouse Monoclonal Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
FZD1 / Frizzled 1 Rat anti-Mouse Monoclonal Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Specifications
Description
Frizzled 1 antibody LS-C6894 is an unconjugated rat monoclonal antibody to Frizzled 1 (FZD1) from mouse. It is reactive with human and mouse. Validated for ELISA, Flow and WB.
Target
Mouse FZD1 / Frizzled 1
Synonyms
FZD1 | Chfz-1 | Fz1 | Frizzled 1 | Fz receptor 1 | HFz1 | Frizzled homolog 1 | Fz-1 | FzE1 | Rfz1 | Xfrizzled-1 | Wnt receptor | Dfrizzled-1 | Frizzled family receptor 1 | Frizzled-1
Host
Rat
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
NS0-derived rmFrizzled-1 CRD.
Specificity
Recognizes mouse Frizzled-1. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7, -8 or rhMFRP. By Flow Cytometry, detects Frizzled-1 on differentiated embryoid bodies derived from the mouse embryonic cell line D3. Also recognizes Frizzled-1 on human HEK 293 cells by Flow Cytometry.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry
- ELISA
Usage
Suitable for use in Western Blot, Flow Cytometry and ELISA. Western Blot: 1-2 ug/ml. With a colorimetric detection system, the detection limit is ~5 ng/lane and ~25 ng/lane under non-reducing and reducing conditions, respectively. Flow Cytometry: 0.1 mg/ml; Add 10 ul to label1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. Visualized by using a secondary antibody (goat anti-rat IgG (FITC)). ELISA (Direct): Can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~10 ng/well.
Presentation
PBS, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FZD1 / Frizzled 1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
