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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FXR1 Antibody (aa317‑330) LS‑C34682
Note: This antibody replaces LS-C66294
FXR1 antibody LS-C34682 is an unconjugated goat polyclonal antibody to FXR1 from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C34682-100
100 µg (0.5 mg/ml)
$405

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Product Description

FXR1 antibody LS-C34682 is an unconjugated goat polyclonal antibody to FXR1 from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.

Specifications

Target
Human FXR1
Synonyms
FXR1, HFXR1p, FXR1P
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
FXR1 antibody was raised against synthetic peptide C-RIEGDNENKLPRED from an internal region of human FXR1 (NP_005078.2; NP_001013456.1; NP_001013457.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%); Xenopus (93%).
Blocking Peptide
LS-E26145 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C34682. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa317-330
Specificity
Human FXR1. This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80 kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 59.9 kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FXR1
RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. P51114 NM_005087 NP_005078.2


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Images

Western blot

FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
FXR1 antibody (0.2 ug/ml) staining of NIH/3T3 cell lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 11/13/2018

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