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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FUT1 / HSC Antibody (aa134‑164) LS‑C407806
HSC antibody LS-C407806 is an unconjugated rabbit polyclonal antibody to HSC (FUT1) from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C407806-100
100 µg
$355

Popular FUT1 / HSC Products

Western blot of FUT1 antibody in CEM cell line lysates (35 ug/lane). FUT1 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot, Flow Cytometry
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Species: Mouse
Applications: Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of Human gastric cancer using FUT1 Polyclonal Antibody at dilution of 1:30.
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry - Paraffin Image
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

HSC antibody LS-C407806 is an unconjugated rabbit polyclonal antibody to HSC (FUT1) from human, mouse and rat. Validated for IHC and WB.

Specifications

Target
Human FUT1 / HSC
Synonyms
FUT1, 2-alpha-L-fucosyltransferase, Alpha (1,2) fucosyltransferase, Alpha(1,2)FT 1, Fucosyltransferase 1, HH, HSC
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FUT1 / HSC antibody was raised against a synthetic peptide corresponding to a sequence at the N-terminus of human FUT1 (134-164aa EVDSRTPWRELQLHDWMSEEYADLRDPFLKL), different from the related mouse and rat sequences by seven amino acids.
Epitope
aa134-164
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 7.1 mM sodium phosphate, 77 mM NaCl, 2.5% BSA, 0.025% sodium azide
Reconstitution
Reconstitute with 200 µl of distilled water.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FUT1 / HSC
Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. P19526 NM_000148 NP_000139.1


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Images

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Western blot

FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.
FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Western blot

FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.
FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Western blot

FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.
FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Rat Intestine Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

Immunohistochemistry

FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
FUT1 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Western blot

FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.
FUT1 antibody Western blot. All lanes: Anti FUT1 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 50 kD. Observed band size: 50 kD.

Requested From: United States
Date Requested: 11/14/2018

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