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Catalog Number Size Price
LS-C344739-50 50 µg $354 
LS-C344739-100 100 µg (1 mg/ml) $407 
FTO Antibody - Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).
FTO Antibody - Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb . 1. hFTO (His-tagged) recombinant control 100ng (AG-40A-112). 2. hFTO (His-tagged) 5 ug. 3. hFTO (His-tagged) 2.5 ug. 4. hFTO (His-tagged) 1.25 ug. 5. hFTO (His-tagged) 0.625 ug. 6. hFTO (His-tagged) 0.312 ug. 7. hFTO (His-tagged) 0.156 ug. 8. hFTO (His-tagged) 0.078 ug
FTO Antibody - Western blot analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:. 1. hFTO (His-tagged) 100 ng. 2. HEK293T 100 ug. 3. HEK293E 100 ug. 4. HepG2 100 ug. 5. Hep3B 50 ug. 6. VIT1 80 ug. 7. THP1 74 ug. 8. Molt4 96 ug. 9. A549 48 ug
FTO Antibody - Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).
FTO Antibody - Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb . 1. hFTO (His-tagged) recombinant control 100ng (AG-40A-112). 2. hFTO (His-tagged) 5 ug. 3. hFTO (His-tagged) 2.5 ug. 4. hFTO (His-tagged) 1.25 ug. 5. hFTO (His-tagged) 0.625 ug. 6. hFTO (His-tagged) 0.312 ug. 7. hFTO (His-tagged) 0.156 ug. 8. hFTO (His-tagged) 0.078 ug
FTO Antibody - Western blot analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:. 1. hFTO (His-tagged) 100 ng. 2. HEK293T 100 ug. 3. HEK293E 100 ug. 4. HepG2 100 ug. 5. Hep3B 50 ug. 6. VIT1 80 ug. 7. THP1 74 ug. 8. Molt4 96 ug. 9. A549 48 ug
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Monoclonal Mouse anti‑Human FTO Antibody (clone AG103, IHC, WB) LS‑C344739

Monoclonal Mouse anti‑Human FTO Antibody (clone AG103, IHC, WB) LS‑C344739

Antibody:
FTO Mouse anti-Human Monoclonal (AG103) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$354
LS-C344739-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FTO Mouse anti-Human Monoclonal (AG103) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FTO antibody LS-C344739 is an unconjugated mouse monoclonal antibody to human FTO. Validated for ELISA, IHC, IP and WB.
Target
Human FTO
Synonyms
FTO | KIAA1752 | Protein fto
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
AG103
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human FTO.
Specificity
Recognizes human FTO.
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation (5 µg/ml)
  • ELISA (1:2000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
ELISA - Direct and indirect: 1:2000-1:5000. Immunoprecipitation - 5 ug/ml per sample in a final reaction volume of 500-1000ul. Immunohistochemistry - 5-10 ug/ml: paraffin sections. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FTO
Q9C0B1 Q9C0B1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024