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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FSTL1 Antibody (aa222‑271) LS‑C117090
FSTL1 antibody LS-C117090 is an unconjugated rabbit polyclonal antibody to FSTL1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C117090-50
50 µg
$365
Description
FSTL1 antibody LS-C117090 is an unconjugated rabbit polyclonal antibody to FSTL1 from human, mouse, rat and other species. Validated for WB.
Target
Human FSTL1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
FSTL1 antibody was raised against synthetic peptide located between aa222-271 of human FSTL1 (Q12841, NP_009016). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Guinea pig (100%); Marmoset, Bat, Zebra finch (92%); Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Zebrafish (85%).
Blocking Peptide
LS-E18791 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C117090. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa222-271
Specificity
Human FSTL1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FSTL1
Q12841 NM_007085 NP_009016.1

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Images

Western blot

FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FSTL1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/17/2018
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