Human FSCN1 / Fascin

Mouse

Human, Rat (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

FSCN1/418

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Full length recombinant human protein.

Human FSCN1 / Fascin

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. Optimal dilution of the Fascin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16658 NCBI: NM_003088 NP_003079.1