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Catalog Number Size Price
LS-C98676-400 400 µl $393 
Fragilis / IFITM3 Antibody - Fluorescent confocal image of HeLa cells stained with IFITM3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.
Fragilis / IFITM3 Antibody - Western blot of lysate from HT1080 cell line,using IFITM3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Fragilis / IFITM3 Antibody - IFITM3 Antibody western blot of HeLa,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35 ug/lane). The IFITM3 antibody detected the IFITM3 protein (arrow).
Fragilis / IFITM3 Antibody - Fluorescent confocal image of HeLa cells stained with IFITM3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.
Fragilis / IFITM3 Antibody - Western blot of lysate from HT1080 cell line,using IFITM3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Fragilis / IFITM3 Antibody - IFITM3 Antibody western blot of HeLa,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35 ug/lane). The IFITM3 antibody detected the IFITM3 protein (arrow).
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Polyclonal Rabbit anti‑Human Fragilis / IFITM3 Antibody (aa1‑30, IF, WB) LS‑C98676

Polyclonal Rabbit anti‑Human Fragilis / IFITM3 Antibody (aa1‑30, IF, WB) LS‑C98676

Antibody:
Fragilis / IFITM3 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98676-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Fragilis / IFITM3 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IFITM3 antibody LS-C98676 is an unconjugated rabbit polyclonal antibody to human IFITM3 (Fragilis) (aa1-30). Validated for ICC, IF and WB. Cited in 1 publication.
Target
Human Fragilis / IFITM3
Synonyms
IFITM3 | 1-8U | Dispanin subfamily A member 2b | DSPA2b | Fragilis | IP15
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa1-30
Specificity
This Interferon-inducible protein (IFITM3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Interferon-inducible protein (IFITM3).
Applications
  • ICC
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Fragilis / IFITM3
Q01628 NM_021034 NP_066362.2

LSBio Ratings

Fragilis / IFITM3 Antibody (aa1-30) for ICC, IF/Immunofluorescence, WB/Western LS-C98676 has an LSBio Rating of
Publications (4)

Publications (1)

IFITM3 inhibits influenza A virus infection by preventing cytosolic entry. Feeley EM, Sims JS, John SP, Chin CR, Pertel T, Chen LM, Gaiha GD, Ryan BJ, Donis RO, Elledge SJ, Brass AL. PLoS pathogens. 2011 7:e1002337.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/24/2024