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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FPR1 / FPR Antibody (aa176‑190) IHC‑plus™ LS‑B2027
Note: This antibody replaces LS-C19408, LS-C66267
FPR antibody LS-B2027 is an unconjugated rabbit polyclonal antibody to human FPR (FPR1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2027-50
50 µg (0.5 mg/ml)
$425
Description
FPR antibody LS-B2027 is an unconjugated rabbit polyclonal antibody to human FPR (FPR1). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FPR1 / FPR
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FPR1 / FPR antibody was raised against a synthetic peptide corresponding to amino acids 176-190 of human FPR1 (CTFNFSPWTNDPKER), GenBank no. NP_002020.1|. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%); Gibbon (93%); Elephant (80%); Mouse (67%); Rat (47%).
Epitope
aa176-190
Specificity
The amino acid sequence used as immunogen is 100% homologous in human, chimp, gorilla and orangutan, and 67% homologous in mouse.
Usage
Immunohistochemistry: LS-B2027 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2027 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About FPR1 / FPR
P21462 NM_002029 NP_002020.1

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Images

Immunohistochemistry

Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.
Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.

Immunohistochemistry

Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.
Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.

Immunohistochemistry

Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.
Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.

Immunohistochemistry

Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-FPR1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Western blot

Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.
Western blot of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using antibody at 1 ug/ml.

Requested From: United States
Date Requested: 12/12/2018
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