FOXP3 antibody LS-C344820 is an unconjugated rat polyclonal antibody to mouse FOXP3. Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1:2000 - 1:5000)
- ELISA (1:2000 - 1:10000)
FOXP3 antibody was raised against recombinant mouse FOXP3.
Recognizes mouse FOXP3. Detects a band of ~46-49kDa by Western blot.
ELISA - Direct and indirect: 1:2000-1:10000. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
PBS, pH 7.4, no preservatives.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.