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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody LS‑C344820
FOXP3 antibody LS-C344820 is an unconjugated rat polyclonal antibody to mouse FOXP3. Validated for ELISA and WB.
Catalog
Size
Price
LS-C344820-100
100 µg
$325
Description
FOXP3 antibody LS-C344820 is an unconjugated rat polyclonal antibody to mouse FOXP3. Validated for ELISA and WB.
Target
Mouse FOXP3
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:5000)
  • ELISA (1:2000 - 1:10000)
Immunogen
FOXP3 antibody was raised against recombinant mouse FOXP3.
Specificity
Recognizes mouse FOXP3. Detects a band of ~46-49kDa by Western blot.
Usage
ELISA - Direct and indirect: 1:2000-1:10000. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

Popular FOXP3 Products

FOXP3 antibody ARP32743_T100-NP_054728-FOXP3 (forkhead box P3) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
IHC of FOXP3 on FFPE Tonsil tissue Intended Use For In Vitro Diagnostic Use.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
Antibody
Species: Mouse
Applications: ICC, Western blot, Flow Cytometry
The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: ICC, Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Flow Cytometry

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Images

Western blot

Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.
Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.

Western blot

Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.
Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.

Western blot

Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.
Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.

Western blot

Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.
Western blot analysis using anti-FOXP3 (mouse), pAb at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treated mouse T cell (CD4+) cell lysate.

Requested From: United States
Date Requested: 12/18/2018
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