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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody (C‑Terminus, DY650) LS‑C142332
FOXP3 antibody LS-C142332 is a DY650-conjugated rabbit polyclonal antibody to human FOXP3. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C142332-100
100 µl
$365
Description
FOXP3 antibody LS-C142332 is a DY650-conjugated rabbit polyclonal antibody to human FOXP3. Validated for ICC, IF, IHC and WB.
Target
Human FOXP3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse
Clonality
Polyclonal
Conjugations
DyLight 650. Also available Unconjugated or conjugated with Biotin, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (1:50)
  • ICC
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXP3 antibody was raised against a synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431)
Epitope
C-Terminus
Specificity
Human FOXP3.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

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FOXP3 antibody ARP32743_T100-NP_054728-FOXP3 (forkhead box P3) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
IHC of FOXP3 on FFPE Tonsil tissue Intended Use For In Vitro Diagnostic Use.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
Antibody
Species: Mouse
Applications: ICC, Western blot, Flow Cytometry
The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: ICC, Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, IHC - Frozen, Flow Cytometry

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Images

Immunofluorescence

Sample: Healthy thymus from a C57BL/6 mouse.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sample: Healthy thymus from a C57BL/6 mouse. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Incubated only with CD4 and no Foxp3.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Incubated only with CD4 and no Foxp3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Sample: Healthy thymus from a C57BL/6 mouse.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sample: Healthy thymus from a C57BL/6 mouse. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Incubated only with CD4 and no Foxp3.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Incubated only with CD4 and no Foxp3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Sample: Healthy thymus from a C57BL/6 mouse.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sample: Healthy thymus from a C57BL/6 mouse. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Incubated only with CD4 and no Foxp3.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Incubated only with CD4 and no Foxp3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Sample: Healthy thymus from a C57BL/6 mouse.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sample: Healthy thymus from a C57BL/6 mouse. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Incubated only with CD4 and no Foxp3.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Incubated only with CD4 and no Foxp3. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3:293/mouse foxp3, lane 4:293/empty vector. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2018
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