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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXP3 Antibody (aa416-427) LS-C54873

Note: This antibody replaces LS-C66368
In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4µg/ml.
Goat Anti-FOXP3 / Scurfin (Mouse) Antibody Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4µg/ml.
Goat Anti-FOXP3 / Scurfin (Mouse) Antibody Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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FOXP3 Antibody (aa416-427) LS-C54873

Note: This antibody replaces LS-C66368
Antibody:
Goat Polyclonal to Mouse FOXP3
Application:
IHC-P, Flo, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405 
LS-C54873-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Mouse FOXP3
Application:
IHC-P, Flo, Peptide-ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
FOXP3 antibody LS-C54873 is an unconjugated goat polyclonal antibody to mouse FOXP3. Validated for Flow, IHC and Peptide-ELISA.

Target
Mouse FOXP3
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC - Paraffin (2 - 4 µg/ml)
  • Flow Cytometry (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FOXP3 antibody was raised against peptide with sequence KRSQRPNKCSNP, from the C Terminus of the protein sequence according to NP_473380.1.
Blocking Peptide
LS-E25726 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54873. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa416-427
Specificity
Mouse FOXP3. Note - anti-mouse companion product of EB05294
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXP3
Q9BZS1 NM_014009 NP_054728.2

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Requested From: United States
Date Requested: 6/26/2019
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