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Catalog Number Size Price
LS-C368596-30 30 µl (1 mg/ml) $253 
LS-C368596-100 100 µl (1 mg/ml) $305 
LS-C368596-200 200 µl (1 mg/ml) $369 
FOXO3 / FOXO3A Antibody - Immunohistochemical analysis of FOXO3 (pS253) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FOXO3 / FOXO3A Antibody - Immunofluorescent analysis of FOXO3 (pS253) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FOXO3 / FOXO3A Antibody - Western blot analysis of FOXO3 (pS253) expression in HeLa (A); Raw264.7 (B); mouse liver (C); rat smooth muscle (D) whole cell lysates.
FOXO3 / FOXO3A Antibody - Immunohistochemical analysis of FOXO3 (pS253) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FOXO3 / FOXO3A Antibody - Immunofluorescent analysis of FOXO3 (pS253) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FOXO3 / FOXO3A Antibody - Western blot analysis of FOXO3 (pS253) expression in HeLa (A); Raw264.7 (B); mouse liver (C); rat smooth muscle (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human FOXO3 / FOXO3A Antibody (phospho‑Ser253, IHC, IF, WB) LS‑C368596

Polyclonal Rabbit anti‑Human FOXO3 / FOXO3A Antibody (phospho‑Ser253, IHC, IF, WB) LS‑C368596

Antibody:
FOXO3 / FOXO3A Rabbit anti-Human Polyclonal (pSer253) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C368596-30
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
FOXO3 / FOXO3A Rabbit anti-Human Polyclonal (pSer253) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
FOXO3A antibody LS-C368596 is an unconjugated rabbit polyclonal antibody to FOXO3A (FOXO3) (pSer253) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
Target
Human FOXO3 / FOXO3A
Synonyms
FOXO3 | AF6q21 | AF6q21 protein | Forkhead box O3 | Forkhead box O3A | FOXO3A | FKHRL1P2 | Forkhead box protein O3 | FOXO2 | FKHRL1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human FOXO3.
Epitope
pSer253
Specificity
Recognizes endogenous levels of FOXO3 (pS253) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXO3 / FOXO3A
O43524 NM_001455 NP_001446.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2021