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Catalog Number Size Price
LS-C368599-30 30 µl (1 mg/ml) $253 
LS-C368599-100 100 µl (1 mg/ml) $305 
LS-C368599-200 200 µl (1 mg/ml) $369 
FOXO1 / FKHR Antibody - Immunohistochemical analysis of FOXO1 (pS329) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FOXO1 / FKHR Antibody - Immunofluorescent analysis of FOXO1 (pS329) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FOXO1 / FKHR Antibody - Western blot analysis of FOXO1 (pS329) expression in HEK293T (A); SHSY5Y (B); NIH3T3 (C); mouse brain (D) whole cell lysates.
FOXO1 / FKHR Antibody - Immunohistochemical analysis of FOXO1 (pS329) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FOXO1 / FKHR Antibody - Immunofluorescent analysis of FOXO1 (pS329) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FOXO1 / FKHR Antibody - Western blot analysis of FOXO1 (pS329) expression in HEK293T (A); SHSY5Y (B); NIH3T3 (C); mouse brain (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human FOXO1 / FKHR Antibody (phospho‑Ser329, IHC, IF, WB) LS‑C368599

Polyclonal Rabbit anti‑Human FOXO1 / FKHR Antibody (phospho‑Ser329, IHC, IF, WB) LS‑C368599

Antibody:
FOXO1 / FKHR Rabbit anti-Human Polyclonal (pSer329) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C368599-30
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
FOXO1 / FKHR Rabbit anti-Human Polyclonal (pSer329) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
FKHR antibody LS-C368599 is an unconjugated rabbit polyclonal antibody to FKHR (FOXO1) (pSer329) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human FOXO1 / FKHR
Synonyms
FOXO1 | FKHR | Forkhead in rhabdomyosarcoma | FOXO1A | FKH1 | Forkhead box protein O1 | Forkhead box protein O1A | Forkhead box O1 | Forkhead box o1a | Forkhead domain protein fkhr
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human FOXO1.
Epitope
pSer329
Specificity
Recognizes endogenous levels of FOXO1 (pS329) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXO1 / FKHR
Q12778 NM_002015 NP_002006.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/5/2021