Target
Human FOXO1 / FKHR
Synonyms
FOXO1 | FKHR | Forkhead in rhabdomyosarcoma | FOXO1A | FKH1 | Forkhead box protein O1 | Forkhead box protein O1A | Forkhead box O1 | Forkhead box o1a | Forkhead domain protein fkhr
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser256 of human FKHR.
Specificity
Detects endogenous levels of total levels of FKHR only when phosphorylated at Ser256. Cross-reacts with phosphorylated AFX. Species cross-reactivity: Human, mouse, rat.
Usage
Suitable for use in Western Blot. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4?C with gentle shaking overnight.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee