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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXI1 Antibody (C‑Terminus) LS‑C80243
FOXI1 antibody LS-C80243 is an unconjugated rabbit polyclonal antibody to FOXI1 from human, mouse, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C80243-50
50 µg
$365

Popular FOXI1 Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-FOXI1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human Kidney tissue using anti-FOXI1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human prostate tissue using anti-FOXI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

FOXI1 antibody LS-C80243 is an unconjugated rabbit polyclonal antibody to FOXI1 from human, mouse, bovine and other species. Validated for WB.

Specifications

Target
Human FOXI1
Synonyms
FOXI1, Forkhead-related activator 6, FREAC6, Fork-head homolog-3, Forkhead-like 10, FREAC-6, HNF-3/fork-head homolog 3, HFH-3, FKHL10, Forkhead box protein I1, HFH3, FKH10, Forkhead box I1, HNF-3/fork-head homolog-3
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Dog, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
FOXI1 antibody was raised against synthetic peptide from C-Terminus of human FOXI1 (Q12951, NP_036320). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Baboon, Monkey, Marmoset (92%); Zebrafish (90%); Gibbon, Galago, Elephant, Panda, Rabbit (85%); Bovine, Platypus (84%); Mouse (78%).
Blocking Peptide
LS-E14045 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80243. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human FOXI1
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About FOXI1
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. Q12951 NM_012188 NP_036320.2


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Images

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/14/2018

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