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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FOXC1 Antibody LS-C355641

Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
WB using FOXC1 Antibody
Enrichment of endogenous FOXC1 Protein at specific gene loci using Anti-FOXC1 Protein Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-FOXC1 Protein Rabbit Polyclonal Antibody on sheared chromatin from 2 million HeLa cells using the ""MAGnify ChIP system"" kit. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the promoter of active FGF gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
WB using FOXC1 Antibody
Enrichment of endogenous FOXC1 Protein at specific gene loci using Anti-FOXC1 Protein Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-FOXC1 Protein Rabbit Polyclonal Antibody on sheared chromatin from 2 million HeLa cells using the ""MAGnify ChIP system"" kit. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the promoter of active FGF gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
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FOXC1 Antibody LS-C355641

Antibody:
Rabbit Polyclonal to Human FOXC1
Application:
IF, WB, ChrIP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$385 
LS-C355641-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FOXC1
Application:
IF, WB, ChrIP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
FOXC1 antibody LS-C355641 is an unconjugated rabbit polyclonal antibody to FOXC1 from human and mouse. Validated for ChrIP, IF and WB.

Target
Human FOXC1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500)
  • Chromatin Immunoprecipitation
Immunogen
FOXC1 antibody was raised against synthetic peptide corresponding to aa423-434 (AVDDPLPDYSLP) of human FOXC1. Percent identity by BLAST analysis: Human (100%); Elephant (92%).
Specificity
The antibody detects FOXC1 from human and mouse samples. It has been successfully used in Western blot procedures. By Western blot, It detects a ~50 kD band representing FOXC1 from HepG2 cells and mouse kidney cells. This antibody also detects a nonspecific band at ~45 kD from HepG2 cells, and at ~60 kD from mouse kidney samples.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXC1
Q12948 NM_001453 NP_001444.2

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Requested From: United States
Date Requested: 5/25/2019
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