FMRP antibody LS-C15265 is an unconjugated mouse monoclonal antibody to FMRP (FMR1) from human, mouse and rat. Validated for ELISA, ICC, IHC and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- IHC - Frozen
- ICC (1:500 - 1:5000)
- Western blot (1:250 - 1:2500)
- ELISA (1:500 - 1:5000)
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FMR1 / FMRP antibody was raised against fusion protein with a full length FMRP (human).
Recognizes human FMRP. The epitope is localized in the N-terminal half of FMRP. Cross-reaction with FXR protein may be detected in cases of high expression of the latter proteins. Species cross-reactivity: mouse.
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and the detection of FMRP on blood smears. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. ELISA: 1:500-1:5000. Immunohistochemistry (Paraffin and Frozen Sections): 1:500-1:5000. Immunocytochemistry: 1:500-1:5000 was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only). Detection of FMRP on Blood Smears: 1:500-1:5000. Positive control: HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate.
No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.