FMR1 / FMRP antibody was raised against fusion protein with a full length FMRP (human).
Recognizes human FMRP. The epitope is localized in the N-terminal half of FMRP. Cross-reaction with FXR protein may be detected in cases of high expression of the latter proteins. Species cross-reactivity: mouse.
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and the detection of FMRP on blood smears. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. ELISA: 1:500-1:5000. Immunohistochemistry (Paraffin and Frozen Sections): 1:500-1:5000. Immunocytochemistry: 1:500-1:5000 was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only). Detection of FMRP on Blood Smears: 1:500-1:5000. Positive control: HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.