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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154035-100 100 µg (1 mg/ml) $425 
fliC / Flagellin Antibody - Anti-Flagellin Antibody - Western Blot. Western blot showing detection of 0.1 ug of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti‑Borrelia burgdorferi fliC / Flagellin Antibody (Lyophilized, WB) LS‑C154035

Polyclonal Rabbit anti‑Borrelia burgdorferi fliC / Flagellin Antibody (Lyophilized, WB) LS‑C154035

Antibody:
Rabbit Polyclonal to Borrelia burgdorferi fliC / Flagellin
Application:
WB, ELISA
Reactivity:
Borrelia burgdorferi
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$425 
LS-C154035-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Borrelia burgdorferi fliC / Flagellin
Application:
WB, ELISA
Reactivity:
Borrelia burgdorferi
Format:
Unconjugated, Lyophilized

Specifications

Description
Flagellin antibody LS-C154035 is an unconjugated rabbit polyclonal antibody to borrelia burgdorferi Flagellin (fliC). Validated for ELISA and WB.
Target
Borrelia burgdorferi fliC / Flagellin
Synonyms
fliC
Host
Rabbit
Reactivity
Borrelia burgdorferi (tested or 100% immunogen sequence identity)
Predicted
Borrelia garinii (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Applications
  • Western blot (1:1000)
  • ELISA (1:6000)
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein.
Specificity
This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined.
Usage
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 33.9 kD in size corresponding to Borrelia burgdorferi Flagellin protein by Western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About fliC / Flagellin
NP_416433.1

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Requested From: United States
Date Requested: 6/6/2020