Clonality
IgG1
Monoclonal
Conjugations
Horseradish Peroxidase.
Also available
Unconjugated or conjugated with
Biotin.
Purification
Purified IgG
Specificity
FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Applications
- Western blot
- ELISA
- Applications tested for the base form of this product only
Usage
This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1000 upwards; in Western blotting from 1:2000 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with 0.5ml distilled H2O.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee