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Catalog Number Size Price
LS-C147253-0.5 0.5 mg $415 
Antibody

FITC Antibody (clone NI 239) LS‑C147253

FITC Antibody (clone NI 239) LS‑C147253

Antibody:
Mouse Monoclonal to FITC
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$415 
LS-C147253-0.5
0.5 mg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to FITC
Application:
WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
FITC antibody LS-C147253 is an unconjugated mouse monoclonal antibody to FITC. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG1 Monoclonal
Clone
NI 239
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
FITC.
Specificity
FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Usage
This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
Presentation
Lyophilized
Reconstitution
Reconstitute with 0.5ml distilled H2O.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/18/2019