Photinus pyralis Firefly Luciferase

Mouse

Photinus pyralis (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Luciferase protein from Photinus pyralis.

This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.

• IHC
• IHC - Frozen
• Immunofluorescence (10 - 30 µg/ml)
• Western blot (1 - 3 µg/ml)

Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 ug/ml. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 ug/ml. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 ug/ml.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08659 NCBI: M15077 P08659