Human Filamin

Mouse

Human, Bovine (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Ascites

Unmodified

Human platelet protein.

Human filamin (actin-binding protein). Recognizes the 90kD fragment of filamin produced by calpain cleavage. Species cross-reactivity: Human and bovine. Does not react with Mouse or rat.

• IHC
• IHC - Paraffin (1:50)
• Immunofluorescence (1:50 - 1:200)
• Western blot (1:250 - 1:1000)
• Immunoprecipitation

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Western Blot: 1:250-1:1000. Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for. every 300-500muL of cell lysate (200-500 ug/mL total protein is suggested. Incubation is 1hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are. recommended for full length filamin or the 80kD fragment visualization. Immunofluorescence:1:50-1:200. ABC. Immunohistochemistry: foramlin-fixed paraffin-embedded sections. Requires HIER (citrate buffer). Positive control: Human, prostate or bovine skin.

No preservatives added

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.