FHIT antibody LS-B3166 is an unconjugated rabbit polyclonal antibody to FHIT from human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (5 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
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FHIT antibody was raised against full length human FHIT fusion protein with GST (glutathione amino transferase).
Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Reacts with human and rat FHIT. Cross-reactivity with rat has been confirmed in WB only. Suitability for use with rat in IHC has not yet been evaluated.
Immunohistochemistry: LS-B3166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3166 was determined to be 5 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.