Human FHIT

Rabbit

Human, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Full length human FHIT fusion protein with GST (glutathione amino transferase)

Detects full length (~17kD) FHIT protein in cell and tissue lysates derived from human and rat in Western blot. It is not reactive with mouse FHIT protein. Reactivity has been confirmed by Western blotting using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Reacts with human and rat FHIT. Cross-reactivity with rat has been confirmed in WB only. Suitability for use with rat in IHC has not yet been evaluated.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot
• ELISA

Immunohistochemistry: LS-B3166 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3166 was determined to be 5 ug/ml.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49789 NCBI: NM_002012 NP_002003.1