Human FGF6

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Human, recombinant Fibroblast Growth Factor 6 (FGF6) from E. coli.

Recognizes human, recombinant Fibroblast Growth Factor 6 (FGF6). ~50% cross-reactivity with rhFGF4, less than 5% cross-reactivity with rhFGF9 and less than 2% cross-reactivity with FGF-acidic, rhFGF5 and rhFGF7. In direct ELISA, this antibody shows no cross-reactivity with other cytokines tested.

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)
• Functional Assay
• Neutralization

Suitable for use in Neutralization, Western Blot and ELISA. Neutralization: ND50 is ~0.025-0.075 ug/ml in the presence of 1 ng/ml of rhFGF6 and 1 ug/ml of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16 ng/well.

PBS, pH 7.2, 40% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10767 NCBI: NM_020996 NP_066276.2