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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FGF3 Antibody (aa160‑209) IHC‑plus™ LS‑B11923
Note: This antibody replaces LS-C135112
FGF3 antibody LS-B11923 is an unconjugated rabbit polyclonal antibody to FGF3 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11923-50
50 µg
$485

Popular FGF3 Products

Antibody
Species: Human, Mouse, Xenopus
Applications: IHC
Western blot of recombinant FGF3.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Antibody
Species: Human
Applications: ICC, Western blot
Antibody
Species: Human
Applications: ICC, Western blot

Product Description

FGF3 antibody LS-B11923 is an unconjugated rabbit polyclonal antibody to FGF3 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About FGF3
FGF3 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. P11487 NM_005247 NP_005238.1

FGF3 Antibody, FGF-3 Antibody, Fibroblast growth factor 3 Antibody, HBGF-3 Antibody, Proto-oncogene Int-2 Antibody, INT-2 proto-oncogene protein Antibody, INT2 Antibody, Oncogene INT2 Antibody


Specifications

Target
Rat FGF3
Host
Rabbit
Reactivity
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FGF3 antibody was raised against synthetic peptide located between aa160-209 of rat Fgf3 (Q8R5L9, NP_570830). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%).
Blocking Peptide
LS-E15533 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11923. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa160-209
Specificity
Rat FGF3
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FGF3 antibody western blot of Rat Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FGF3 antibody western blot of Rat Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FGF3 antibody western blot of Rat Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FGF3 antibody western blot of Rat Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FGF3 antibody western blot of Rat Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FGF3 antibody western blot of Rat Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

FGF3 antibody western blot of Rat Liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
FGF3 antibody western blot of Rat Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/24/2018

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