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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FGF1 / Acidic FGF Antibody (clone 2E12) IHC-plus™ LS-B5406

Note: This antibody replaces LS-C133186
Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of monoclonal antibody to FGF1 on HeLa cell. [antibody concentration 10 ug/ml].
Western blot of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody clone 2E12.
Detection limit for recombinant GST tagged FGF1 is approximately 10 ng/ml as a capture antibody.
Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of monoclonal antibody to FGF1 on HeLa cell. [antibody concentration 10 ug/ml].
Western blot of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody clone 2E12.
Detection limit for recombinant GST tagged FGF1 is approximately 10 ng/ml as a capture antibody.
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FGF1 / Acidic FGF Antibody (clone 2E12) IHC-plus™ LS-B5406

Note: This antibody replaces LS-C133186
Antibody:
Mouse Monoclonal to Human FGF1 / Acidic FGF
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495 
LS-B5406-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human FGF1 / Acidic FGF
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Acidic FGF antibody LS-B5406 is an unconjugated mouse monoclonal antibody to human Acidic FGF (FGF1). Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human FGF1 / Acidic FGF
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
2E12
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FGF1 (AAH32697, 46 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human FGF1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FGF1 / Acidic FGF
P05230 X59065 P05230

Validation

Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FGF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 7/19/2019
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