FEN1 | DNase IV | FEN-1 | HFEN-1 | Flap endonuclease 1 | MF1 | RAD2 | Maturation factor 1 | Maturation factor-1
(tested or 100% immunogen sequence identity)
Synthetic peptide corresponding to a region within the N-Terminus domain of human FEN1 (locus link ID 2237).
Recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45kD member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair. It is hypothesised that FEN1 may play a role in the inhibition of triplet repeat expansion. Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against whichis generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAse activity. Initial results suggest thatdoes not recognize the truncated form of human FEN1. Fen1 is expressed in the nucleus of a wide variety of organ structures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Recognizes a band of ~45kD in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.