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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FcERI / Fc Epsilon RI Antibody (clone MAR‑1, FITC) LS‑C105809
Fc Epsilon RI antibody LS-C105809 is an FITC-conjugated hamster-armenian monoclonal antibody to mouse Fc Epsilon RI (FcERI). Validated for Flow.
Catalog
Size
Price
LS-C105809-50
50 µg
$225
LS-C105809-100
100 µg
$275
LS-C105809-500
500 µg
$395

Popular FcERI / Fc Epsilon RI Products

Anti-FCER1A antibody IHC of human mast cell in human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Surface staining of MC/9 cells with anti-mouse FceRI alpha (MAR-1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Species: Mouse
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Antibody
Species: Rat
Applications: Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Product Description

Fc Epsilon RI antibody LS-C105809 is an FITC-conjugated hamster-armenian monoclonal antibody to mouse Fc Epsilon RI (FcERI). Validated for Flow.

Specifications

Target
Mouse FcERI / Fc Epsilon RI
Synonyms
FCER1A, Alpha polypeptide, FCE1A, FcERI, Fc Epsilon RI, Fc epsilon RI alpha-chain, Fc-epsilon RI-alpha, IgE Fc receptor subunit alpha
Host
Hamster-Armenian
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [MAR-1]
Conjugations
FITC. Also available Unconjugated or conjugated with PE, Biotin.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
Mouse FCER1A
Usage
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About FcERI / Fc Epsilon RI
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. P12319 NM_002001 NP_001992.1


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Images

Flow Cytometry

Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control  (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control  (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control  (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control  (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flo Staining of MC/9 cells with 0.06 ug of FITC Armenian hamster IgG isotype control (open histogram) or 0.06 ug of FITC MAR-1 (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/14/2018

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