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Catalog Number	Size	Price
LS-C313532-10	10 µg	$318
LS-C313532-100	100 µg (0.5 mg/ml)	$470

FCER2 / CD23 Antibody - IHC analysis of CD23 using anti-CD23 antibody. CD23 was detected in frozen section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD23 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

FCER2 / CD23 Antibody - Immunohistochemistry - Anti-CD23/FCER2 Antibody

FCER2 / CD23 Antibody - IF analysis of CD23 using anti-CD23 antibody CD23 was detected in paraffin-embedded section of rat lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD23 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

FCER2 / CD23 Antibody - IF analysis of CD23 using anti-CD23 antibody CD23 was detected in paraffin-embedded section of mouse lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD23 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

FCER2 / CD23 Antibody - Western blot analysis of CD23 using anti-CD23 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD23 at approximately 49KD. The expected band size for CD23 is at 36KD.

FCER2 / CD23 Antibody - Western blot - Anti-CD23/FCER2 Antibody

FCER2 / CD23 Antibody - Flow Cytometry analysis of mouse PBMC cells using anti-CD23 antibody. Overlay histogram showing mouse PBMC cells stained with anti-CD23 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD23 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

FCER2 / CD23 Antibody - Flow Cytometry analysis of mouse BMC cells using anti-CD23 antibody. Overlay histogram showing mouse BMC cells stained with anti-CD23 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD23 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Mouse FCER2 / CD23 Antibody (aa50‑331, IHC, WB) LS‑C313532

Name: Anti-FCER2 / CD23 Antibody | Rabbit anti-Mouse Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313532-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

FCER2 / CD23 Rabbit anti-Mouse Polyclonal (aa50-331) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Human, Rat

Format:

Unconjugated, Unmodified

$318

LS-C313532-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FCER2 / CD23 Rabbit anti-Mouse Polyclonal (aa50-331) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CD23 antibody LS-C313532 is an unconjugated rabbit polyclonal antibody to CD23 (FCER2) (aa50-331) from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Mouse FCER2 / CD23

Synonyms

See All FCER2 / CD23 Antibodies

Host

Rabbit

Reactivity

Mouse, Human, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23.

Epitope

aa50-331

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FCER2 / CD23

Uniprot: P06734 NCBI: NM_002002 NP_001993.2