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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FBL / FIB / Fibrillarin Antibody (clone 38F3) LS‑C2552 Best Seller
Note: This antibody replaces LS-C66045
Fibrillarin antibody LS-C2552 is an unconjugated mouse monoclonal antibody to Fibrillarin (FBL / FIB) from human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C2552-250
250 µl
$335

Popular FBL / FIB / Fibrillarin Products

FBL / Fibrillarin antibody LS-C30767 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
Applications: IHC, IHC - Paraffin, Western blot
Human SH-SY5Y cells stained with FBL / FIB / Fibrillarin antibody, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. The cells are also stained with chicken antibody to neurofilament NF-H.
Species: Yeast, Human, Rat
Applications: ICC, Immunofluorescence, Western blot
FBL antibody (1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
FBL antibody (0.1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Xenopus, Zebrafish
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Fibrillarin antibody LS-C2552 is an unconjugated mouse monoclonal antibody to Fibrillarin (FBL / FIB) from human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.

Specifications

Target
Rat FBL / FIB / Fibrillarin
Synonyms
FBL, FLRN, FBRL, FIB1, Fibrillarin, RNU3IP1, FIB
Host
Mouse
Reactivity
Rat, Human, Mouse, Chicken, Drosophila, Yeast, C. elegans, Plant (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [38F3]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Yeast nuclear preparation (S. cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae)
Specificity
Fibrillarin (38F3) - Nuclear Marker.
Presentation
Tissue Culture Supernatant, 10 mM Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About FBL / FIB / Fibrillarin
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. P22087 NM_001436 NP_001427.2


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Images

Immunofluorescence

Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.

Immunofluorescence

Mouse fibrillarin Hoechst DNA detected in Hek293 cells.
Mouse fibrillarin Hoechst DNA detected in Hek293 cells.

Western blot

Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.
Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.

Immunofluorescence

Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.

Immunofluorescence

Mouse fibrillarin Hoechst DNA detected in Hek293 cells.
Mouse fibrillarin Hoechst DNA detected in Hek293 cells.

Western blot

Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.
Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.

Immunofluorescence

Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.

Immunofluorescence

Mouse fibrillarin Hoechst DNA detected in Hek293 cells.
Mouse fibrillarin Hoechst DNA detected in Hek293 cells.

Western blot

Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.
Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.

Immunofluorescence

Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
Human SH-SY5Y cells stained, showing prominent specular nucleolar staining. Note: The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.

Immunofluorescence

Mouse fibrillarin Hoechst DNA detected in Hek293 cells.
Mouse fibrillarin Hoechst DNA detected in Hek293 cells.

Western blot

Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.
Strip blots of yeast protein extracts stained with the indicated antibodies; Is first lane on the left and stains a single band at ~34kD.

Requested From: United States
Date Requested: 10/18/2018

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