Human FASLG / Fas Ligand

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

13 AA peptide from human FASL near the C-Terminus (KLH).

C-Terminus

Recognizes human FASL. Species sequence homology: mouse and rat-92%.

• Western blot (1:1000 - 1:5000)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000 (ECL). We recommend the use of 0.5-1% milk in diluents used for primary antibody and secondary antibody-enzyme conjugates to suppress background. Rat, mouse and human FASL have 278, 279, and 281aa residues, respectively (Calculated MW of ~32kD). Western blot of the oocyte has detected a specific 31-kD band. A 40kD FASL has been detected in mouse T cells. An alternate detecting reagent, mFAS-Fc also detected a cell surface protein of about 40kD. ELISA: 0.5-1 ug/ml.

100 mM Tris, pH 7.5. No stabilizing proteins added.

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P48023 NCBI: NM_000639 NP_000630.1