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Catalog Number Size Price
LS-C745314-25 25 µl (1.1 mg/ml) $275 
FAP Alpha Antibody - Western blot using the affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.
FAP Alpha Antibody - ELISA results of purified Rabbit anti-Fibroblast Activation Protein (FAP) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
FAP Alpha Antibody - Western blot using the affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.
FAP Alpha Antibody - ELISA results of purified Rabbit anti-Fibroblast Activation Protein (FAP) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
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Polyclonal Rabbit anti‑Mouse FAP Alpha Antibody (WB) LS‑C745314

Polyclonal Rabbit anti‑Mouse FAP Alpha Antibody (WB) LS‑C745314

Antibody:
FAP Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745314-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
FAP Alpha Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
FAP Alpha antibody LS-C745314 is an unconjugated rabbit polyclonal antibody to mouse FAP Alpha. Validated for ELISA and WB.
Target
Mouse FAP Alpha
Synonyms
FAP | DPPIV | FAP alpha | Fibroblast activation protein | FAPA | Seprase
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
Specificity
This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence.  Reactivity with FAP from other sources has not been determined.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FAP Alpha
Q12884 NM_004460 NP_004451.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021