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Catalog Number Size Price
LS-C148268-50 50 µl $335 
FAP-1 / PTPN13 Antibody - Immunohistochemistry of FAP-1 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray using Polyclonal Antibody to FAP-1 at 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-Eosin counterstain.
FAP-1 / PTPN13 Antibody - Immunocytochemistry of FAP-1 in cell lines using Polyclonal Antibody to FAP-1 at 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (figure to the left).
FAP-1 / PTPN13 Antibody - Western blot of FAP-1 using Polyclonal Antibody to FAP-1 at 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
FAP-1 / PTPN13 Antibody - Immunohistochemistry of FAP-1 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray using Polyclonal Antibody to FAP-1 at 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-Eosin counterstain.
FAP-1 / PTPN13 Antibody - Immunocytochemistry of FAP-1 in cell lines using Polyclonal Antibody to FAP-1 at 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (figure to the left).
FAP-1 / PTPN13 Antibody - Western blot of FAP-1 using Polyclonal Antibody to FAP-1 at 1:2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
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FAP‑1 / PTPN13 Antibody (aa1279‑1883) LS‑C148268

FAP‑1 / PTPN13 Antibody (aa1279‑1883) LS‑C148268

Antibody:
Rabbit Polyclonal to Human FAP-1 / PTPN13
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C148268-50
50 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human FAP-1 / PTPN13
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PTPN13 antibody LS-C148268 is an unconjugated rabbit polyclonal antibody to human PTPN13 (FAP-1 ). Validated for IHC, IP and WB. Cited in 1 publication.
Target
Human FAP-1 / PTPN13
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant protein corresponding to aa 1279-1883 of human FAP-1 protein, GenBank no. NP_542414.1. This peptide sequence is conserved in multiple isoforms of FAP-1. These FAP-1 isoforms are of varying amino acid (aa) lengths and include: (1) NP_542414.1 = 2485 aa, FAP-1 isoform 1. (2) BAD92141.1 = 2434 aa, FAP-1 isoform 2. (3) NP_542415.1 = 2294 aa, FAP-1 isoform 3. (4) NP_542416.1 = 2490 aa, FAP-1 isoform 4. (5) AAH39610.1 = 604 aa, FAP-1. (6) AAF63474.1 =499 aa, FAP-1.
Epitope
aa1279-1883
Specificity
FAP-1 typically migrates at ~270 kD on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights. The actual molecular weight of FAP-1 observed depends on the isoform expression.
Presentation
Antiserum, 0.05% sodium azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FAP-1 / PTPN13
Q12923 NM_006264 NP_006255.1

Publications (1)

The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation. Wang Y, Hall RA, Lee M, Kamgar-Parsi A, Bi X, Baudry M. Scientific reports. 2017 7:11771. (WB; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/18/2019