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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FAM20A Antibody (aa48-77) for IHC, WB/Western, Flow LS-C167897

FAM20A antibody LS-C167897 is an unconjugated rabbit polyclonal antibody to human FAM20A. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C167897-400
400 µl
$305
Description
FAM20A antibody LS-C167897 is an unconjugated rabbit polyclonal antibody to human FAM20A. Validated for Flow, IHC and WB.
Target
Human FAM20A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7670 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167897. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa48-77
Specificity
This FA20A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the N-terminal region of human FA20A.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About FAM20A
Q96MK3 AL133105 Q96MK3

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Images

Immunohistochemistry

FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.
Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.

Flow Cytometry

FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.
Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.

Flow Cytometry

FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.
Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.

Flow Cytometry

FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
FA20A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human breast carcinoma tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.
Western blot of FA20A Antibody in MCF-7 cell line lysates (35 ug/lane). FA20A (arrow) was detected using the purified antibody.

Flow Cytometry

FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FA20A Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 1/18/2019
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