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| Catalog Number | Size | Price |
|---|---|---|
| LS-C15117-200 | 200 µg | $872 |
Polyclonal Rabbit anti‑Human FAK / Focal Adhesion Kinase Antibody (aa748‑1053, WB) LS‑C15117
Polyclonal Rabbit anti‑Human FAK / Focal Adhesion Kinase Antibody (aa748‑1053, WB) LS‑C15117
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
Focal Adhesion Kinase antibody LS-C15117 is an unconjugated rabbit polyclonal antibody to Focal Adhesion Kinase (FAK) (aa748-1053) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IP and WB.
Target
Human FAK / Focal Adhesion Kinase
Synonyms
PTK2 | FADK | Fadk1 | Focal adhesion kinase | FRNK | FADK 1 | Focal adhesion kinase 1 | FAK1 | p125FAK | PPP1R71 | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | FAK | pp125 FA kinase | Pp125FAK
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
PGEX-derived fusion protein containing aa748-1053 of human FAK.
Epitope
aa748-1053
Specificity
Recognizes human p125 FAK ; does not cross-react with Pyk2. Species cross-reactivity: mouse, rat and hamster not avian.
Applications
- ICC (10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
WB: Detects FAK in RIPA lysates from murine 3T3 and previously in human A431 RIPA cell lysate. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with Rabbit Anti-FAK (1 ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. ICC: Shows positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde. IP: 4ug immunoprecipitates FAK from 500ug of murine 3T3 RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FAK / Focal Adhesion Kinase
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Featured Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Monkey, Mouse
Applications:
IHC, IHC - Paraffin
Species:
Chicken, Human, Rodent
Applications:
Immunofluorescence, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/20/2024
Date Requested: 4/20/2024
