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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FACL2 / ACSL1 Antibody IHC-plus™ LS-B8879

Best Seller
Note: This antibody replaces LS-C153727
ACSL1 antibody LS-B8879 is an unconjugated rabbit polyclonal antibody to ACSL1 (FACL2) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8879-0.05
0.05 mg
$425
Description
ACSL1 antibody LS-B8879 is an unconjugated rabbit polyclonal antibody to ACSL1 (FACL2) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FACL2 / ACSL1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FACL2 / ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1. The immunogen is located within amino acids 240 - 290 of ACSL1.
Blocking Peptide
LS-E31352 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B8879. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.
Usage
ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 41, 58, 77 kDa Observed: 45, 47 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About FACL2 / ACSL1
P33121 NM_001995 NP_001986.2

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Images

Immunohistochemistry

Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.
Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.
Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.
Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FACL2 / ACSL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.
Immunofluorescence of ASCL1 in human lung tissue with ASCL1 antibody at 20 ug/ml.

Western blot

Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of ACSL1 in human lung tissue lysate with ACSL1 antibody at (A) 1 and (B) 2 ug/ml.

Requested From: United States
Date Requested: 2/22/2019
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